| Pos. | First String | Second String | Third String | |||
| QB | 8 | Jon Kitna | 6 | Dan Orlovsky | 5 | Drew Stanton |
| HB | 34 |
Kevin Smith |
32 | Rudi Johnson |
33 | Marcus Thomas |
| FB | 45 |
Jerome Felton |
||||
| WR | 11 | Roy Williams | 84 |
Shaun McDonald |
||
| WR | 81 | Calvin Johnson | 87 | Mike Furrey | ||
| TE | 89 | Dan Campbell |
86 | Michael Gaines |
82 | Casey FitzSimmons |
| LT | 76 | Jeff Backus | 74 | Damion Cook |
||
| LG | 64 | Edwin Mulitalo | ||||
| C | 51 | Dominic Raiola | 67 |
Andy McCollum |
||
| RG | 66 | Stephen Peterman |
63 | Manny Ramirez | ||
| RT | 72 | George Foster | 77 | Gosder Cherilus | ||
| Pos. | First String | Second String | Third String | |||
| LE | 95 | Jared DeVries | 97 | Ikaika Alama-Francis | ||
| NT | 91 | Chuck Darby | 75 | Shaun Cody | 98 | Landon Cohen |
| UT | 78 | Cory Redding | 79 | Langston Moore | 96 | Andre Fluellen |
| RE | 99 | Dewayne White | 93 | Corey Smith | 92 | Cliff Avril |
| WLB | 50 | Ernie Sims | 54 |
Gilbert Gardner |
||
| MLB | 53 | Paris Lenon | 57 |
Jordon Dizon |
||
| SLB | 59 | Alex Lewis | 55 |
Ryan Nece |
||
| LCB | 25 | Brian Kelly | 23 | Keith Smith | ||
| RCB | 21 | Travis Fisher | 28 | Leigh Bodden | 38 | Ramzee Robinson |
| FS | 42 | Gerald Alexander | 24 | Kalvin Pearson | ||
| SS | 26 | Dwight Smith | 27 |
Daniel Bullocks |
||
| Pos. | First String | Second String | Third String | |||
| K | 4 | Jason Hanson | ||||
| P | 2 | Nick Harris | ||||
| H | 2 | Nick Harris | ||||
| LS | 48 | Don Muhlbach | ||||
| PR | 84 |
Shaun McDonald |
||||
| KR | 87 | Mike Furrey |
||||
(John Owens is the fourth tight end on the depth chart)